KIF1A Repeats Cycle of ‘FREE Diffusion’ and ‘SPECIFIC Binding’ during Weak Binding State

Monte Carlo Free Ligand Diffusion with Markov State Model Analysis and Absolute Binding Free Energy Calculations.

Obtaining absolute binding free energies from unbiased ligand diffusion has attracted a significant amount of attention due to its implications in drug design. Several studies have used special purpose computers and software to achieve microsecond molecular dynamics which, combined with a Markov state model analysis, are capable of providing absolute binding free energies. We have recently deve...

A pollen-specific novel calmodulin-binding protein with tetratricopeptide repeats.

Calcium is essential for pollen germination and pollen tube growth. A large body of information has established a link between elevation of cytosolic Ca(2+) at the pollen tube tip and its growth. Since the action of Ca(2+) is primarily mediated by Ca(2+)-binding proteins such as calmodulin (CaM), identification of CaM-binding proteins in pollen should provide insights into the mechanisms by whi...

Expression and Distribution of Calcium-Binding Protein S100P in Human Placenta during Pregnancy

Binding and Diffusion, during Activation of Frog Skeletal Muscle

Cannell and Allen (1984. Biophys. J . 45:913–925) introduced the use of a multi-compartment model to estimate the time course of spread of calcium ions (Ca 2 1 ) within a half sarcomere of a frog skeletal muscle fiber activated by an action potential. Under the assumption that the sites of sarcoplasmic reticulum (SR) Ca 2 1 release are located radially around each myofibril at the Z line, their...

Manganese and Iron Binding to Human Transferrin

The characteristics of manganese and iron binding to human apotransferrin (apo-tf) have been investigated and compared in this study. Both metal ions were taken up by human apo-tf and formed complexes, with the maximum absorbances observed at 410 and 340 nm for manganese-transferrin (Mn-tf) and 465 nm for iron-transferrin (Fe-tf). Addition of manganese (1.5 µg/ml) to the reaction mixture contai...